Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Equilibrative Nucleoside Transporter 1 in human cerebellum, human breast cancer, and human cerebral cortex tissue.

This Anti-Equilibrative Nucleoside Transporter 1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Equilibrative Nucleoside Transporter 1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Equilibrative Nucleoside Transporter 1 (ENT1), also known as Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Equilibrative NBMPR-sensitive nucleoside transporter, Nucleoside transporter, es-type, or Solute carrier family 29 member 1, and encoded by the gene SLC29A1/ENT1, is a transport membrane protein that is responsible for mediating the transport of nucleoside molecules in and out of cells. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. ENT1 is spatially localized to the basolateral cell membranes and is found expressed in many organs including heart, brain, mammary glands, liver and spleen. It is up regulated in cancer cells but it is also an anti-cancer target as it is sensitive to the chemotherapeutic drugs dipyridamole and dilazep.

Immunogen

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Equilibrative Nucleoside Transporter 1. Immunogen is 80% identical to mouse and rat ENT1.

Linkage

Replaces: AB15267

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Equilibrative Nucleoside Transporter 1 in 10 µg of human heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed